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文献和实验Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast) Principle Differential interference microscopy requires several optical components, therefore it can be very expensive to set up. Light from an incandescent
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
until bromophenyl blue is about 3/4 of the way to the bottom; ~1.5 hrs for a 10% gel). 2. Visualize the oligonucleotides using UV shadowing. Place the gel on saran wrap and place on top of an intensifying screen. Shining short wave UV light from a hand held lamp
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