SUMAX400赛默飞转头配件Thermo Fisher S

A safe method of extracting DNA from Coccidioides immitis

in the saprophytic phase at 37 C in 100 ml of 2X GYE medium (20 g glucose and 10 g yeast extract in 1 liter medium, autoclaved for 15 min.) in 500 ml plastic Nalgene flasks with plastic foam stoppers (Identi-plug, Fisher). When there has been sufficient growth

Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)

, but you may find the effect to be more dramatic. The cilia themselves can be seen if you get the paramecium to quiet down. If they won't settle right away, set the slide aside and try again later. Cilia show up best in phase contrast (400x), or in dark field at 100

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge