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文献和实验the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
to 37°C and 65°C Sonicator Spectrophotometer Thermal cycler, automated Timer Tubes, microcentrifuge, 1.5-mL Tubes, polypropylene, 14-mL (e.g., 17- x 100-mm) for use in high-speed centrifuge Tubes, polypropylene, 50-mL
) H2 O, DEPC-treated Heparin (100 mg/mL stock) (Heparin Na-salt; e.g., SERVA 24590.01) HEPES (50 mM, pH 7.5), DEPC-treated M9 salt solution NaOAc (sodium acetate; 3 M, pH 5.2), DEPC-treated Nematode growth media
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