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文献和实验V294.PART II，Chapter 5 细胞粘附实验（英文版）
(see Note 2). 5. Add 100 μL of the cell suspension to each well of the fibronectin-coated ELISAplates and incubate for 60 min in a 37°C incubator under a 5% CO2–95% airatmosphere (see Note 3). 6. Rinse the wells three times with PBS (see Note
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
ng/ml sodium selenite, and 5 µg/ml insulin (Invitrogen). The cell lines were cultivated in Nunc (Thermo Fisher Scientific) cultivation dishes, flasks, and plates in a humidified incubator at 37°C in an atmosphere of 5% CO2 . Real
0.1% SDS 50mM Tris pH 7.4-7.5 150mM NaCl 10% glycerol 1mM EDTA Modified RIPA Buffer - Good general-purpose buffer 1% NP40 0.5% NaDOC 50mM Tris pH 7.5 150mM NaCl 5mM EDTA NP40 Buffer
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