03998赛默飞离心机附件Thermo Fisher ROT

Procedures for In Vitro Production of Bovine Embryos

% (1:1with Sperm-TL) over 3 ml 90% -HEPES-TALP (tight cap and place at 38.5°C) 3-centrifuge tubes with 15 ml HEPES-TALP - IVF-TALP (leave cap loose and place at 38.5°C) 4-well plates (600 ml/well) 1-centrifuge tube with ~5 ml IVF

Nucleolar Isolation Protocol

with ice-cold PBS at 218 g (1000 rpm, Beckman GS-6 centrifuge, GH-3.8 rotor) at 4oC. 4. After the final PBS wash, resuspend the cells in 5ml of Buffer A and incubate the cells on ice for 5 min. Put a small drop of the cell suspension on a glass slide

Methods for DNA isolation

or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture