75005705赛默飞水平转头Thermo Fisher T

核酸电泳工作流程——5 大主要步骤

), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360

Purification of GST Fused Proteins

mM DTT/1%TX-100 and place on wheel for 30 min. Wash 2X in PBS/10mM DTT/1%TX-100. Alternatively, use pre-swollen Pharmacia Glutathione Sepharose beads. These can be washed rapidly 3 times in PBS/10mM DTT/1%TX100. Pellet debris 20 min

A quick RNA mini-prep for Neurospora mycelial cultures

thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed