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文献和实验Preparation of total RNA from whole blood collected in PAXgene tubes
Short PAXgene Protocol:spin in step 6 increased to 20 min; no Dnase treatment; optional wash after step 12 REQUIRED1.Centrifuge the PAXgene Blood RNA Tube for 10 min at 30005000 x g using a swing-out rotor.Note: Ensure that the blood sample
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
itself and thus have virtually no major mechanical failures, other than potential metal stress, which all rotors undergo. These rotors are the work-horse elements of a cell laboratory, and the most common is a rotor holding 8 centrifuge tubes at an angle of 34 ° C
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