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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
solution ( 36.5%; Sigma-Aldrich 33220) Glycine (2 M; Fisher BP381) Glycogen (Roche) H2 O, nuclease-free Immune complex wash buffers (high-salt and low-salt) Prepare the high-salt and low-salt versions of this buffer
Paper GB002 (Schleicher %26amp; Schuell, 34550) Gel Blot Paper GB004 (Schleicher %26amp; Schuell, 34340) Whatman paper, 3MM Contrad 70 detergent (Fisher, 04-355-1) 8 x 10 film cassette (Fisher, FB-XC-810) 14 x 17 film cassette (Fisher, FB-XC-1417 8 x 10
A quick RNA mini-prep for Neurospora mycelial cultures
thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed
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