88881136赛默飞27x22mm固定试管架Thermo

A quick RNA mini-prep for Neurospora mycelial cultures

at 3000g, 4°C in a fixed angle rotor. Make sure caps are screwed on tightly to prevent tubes from collapsing. 8. Pass supernatant through 50 micron Miracloth (Calbiochem #475855, La Jolla, CA) in a funnel into fresh Sarstedt tube. 9. Measure

Procedures for In Vitro Production of Bovine Embryos

OF FERTILIZED OOCYTES INTO EMBRYO CULTURE DROPS (8-10 h post IVF)Materials and Equipment Needed Vortexer Timer 1.5 ml Dolphin microcentrifuge tube and rack X-plate (with prewarmed HEPES-TALP) Plastic Pasteur pipet Heater (placed in front of the microscope) Slide

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

(from Step 24). Incubate with rotation for 3 h at 4°C. 27. Prechill one 1.5-mL tube for each immunoprecipitate. 28. Transfer ~1.5 mL of each immunoprecipitate to a separate prechilled tube. Place the tubes in the magnetic