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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
the medium and resuspend the cells in Chemotaxis Buffer at 0.5 to 1 X 106 cells/ml. Maintain cells at 37°C until the chemotaxis chambers are ready to use. C. Preparation of Agonist Solution(s) 1. Dilute the agonists (chemokines or other chemoattractants
V294.PART II，Chapter 2 Boyden法体外趋化试验
to other stimuli. 2. Materials 1. Standard 48-well chemotaxis chamber (see Fig. 1), including: a lower chamber,a silicone gasket, an upper chamber, and thumb nuts (Neuro Probe; Cabin John,MD). 2. Poretics® polycarbonate: polyvinylpyrrolidone-free
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