76000923赛默飞毛细离心管Thermo Fisher

AFFINITY PURIFICATION OF ANTIBODIES

volume 100mM phosphate, pH 6.2. 6. Add BSA-peptide and react ON with mixing. 7. Wash beads extensively. 8. Pack into a 3ml syringe as column. Add azide! Affinity Purification 1. Equilibrate column with PBS, pH 7.4. 2. Load on sample diluted

单细胞基因表达分析

triethylamine acetate (TEAA, Cat. No. 400613) were from Applied Biosystems and the anhydrous acetonitrile (Cat. No. 40-4050-50) was from Glen Research. Trifluoroacetic acid (TFA, Cat. No. BP618-500) was from Fisher and triethylamine (TEA, Cat. No. T-0886

Single Cell Gene Expression Profiling: Multiplexed Expression Fluorescence in situ Hybridization (FISH)

) was from Fisher and triethylamine (TEA, Cat. No. T-0886) was from Sigma. Fluorophores were purchased from Amersham (Cy3, Cat. No. PA23001; Cy3.5, Cat. No. PA23501; Cy5, Cat. No. PA25001) and Molecular Probes (Oregon Green 488, Cat. No. O-6147; Alexa Fluor 488