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文献和实验AFFINITY PURIFICATION OF ANTIBODIES
(1:10 ?)to run slowly ON (e.g., 160ml @ 8ml/h = 20h). 3. Rinse column: 50ml 2M NaCl, pH 7.5 50ml 0.1M Borate, pH 9.1 50ml 0.1M PBS, pH 4.5 4. Elute antibody with 20mM glycine-HCl/200mM NaCl. Collect 0.8ml fractions into tubes containing 0.4ml
bits, use clean strokes and avoid mashing or tearing the tissue. Add 4ml media and transfer 2ml into the centrifuge tube and leave 4ml in the dish. Gently rock the p-dish to distribute the media. Place the p-dish in the incubator for 2 - 3 days
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
tetracyclin (or NZY tetracyclin) plate. (for optimum results, restreak the cells each week) In a sterile 50ml conical tube, inoculate 10ml of LB media with 12.5µg/ml tetracycline with one or two colonies from the plate. Grow cells to late log phase
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