76002902赛默飞离心机附件Thermo Fisher

Karyotyping of X. tropicalis

  We have been karyotyping animals in the Grainger lab to confirm that the stocks maintained in the lab are indeed X. tropicalis and to establish that wild-caught animals being used for gynogenesis are also X. tropicalis . We recently

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis

用全血直接分选 CD3+ T 细胞

； 1.5 稀释全血，全血：Buffer CI = 3:1。例如：9 ml 外周血加入 3 ml Buffer CI 进行稀释，混匀。为了移除团块，可将稀释后的全血过 40µm 的筛网；对于 PBMCs 样本，用 Buffer CI 重悬 PBMCs 至浓度为：3x108 cells/ 5 ml 1.6 移除柱子上的帽子，剪掉柱子下端。将柱子放置于 Adapter 上，让柱子中的液体流尽； 1.7 柱子中加入 5 ml 的 Buffer CI，完全流尽，下面用离心管接住； 1.8 往柱子中加入 1 ml