B1A-8赛默飞配件（样品梳）Thermo Fisher H

基于PCR技术的染色质沉淀分析

(optional; see Steps 21 and 37) Horizontal gel electrophoresis tank for agarose gels (for duplex PCR only; see Steps 40-53) Standard DNA sequencing gel apparatus with 31 x 38.5-cm glass plates, 0.4-mm spacers, and a shark's-tooth comb (for hot-stop PCR

SDS-PAGE

thick gels or one 1.5 mm gel627 µl 30% acryl:bis780 µl 1M Tris pH 6.85 ml H2031 µl 20% SDS62 µl 10% AP3.8 µl TEMEDPour in between plates, or squirt in with a pasteur pipette. Carefull not to get airbubbles trapped around the teeth of the comb

小RNA的Northern BLOT

while pouring. Tap the glass to get rid off bubbles. Insert comb and slowly lower the plates down to almost horizontal (put two pipette tip boxes under gel). Pour more gel around comb, wait a few minutes and then try to position the gel horizontal. Leave