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- Thermo Fisher
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- 2025年12月31日
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文献和实验Polyclonal and Monoclonal Antibodies
are immunized essentially as described above. Injections: 1. First in Freund's complete 100-200ug/1ml injection, two sites (over each shoulder). 2. Second 1 month later in Freund's INCOMPLETE as above. 3. Bleed 2 weeks later. Boost 2 weeks
. 0.1% SDS. Keep a squirt bottle of it.VIII. Running buffer: Keep a jug of this. 24 g Tris base, 115.2 g glycine, 20 ml 20% SDS, H2O to 4 liters.IX. Sample buffer: 100 mM Tris, pH 6.8, 2% SDS, 5% ß- mercaptoethanol, 15% glycerol, enough bromophenol blue
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
solution ( 36.5%; Sigma-Aldrich 33220) Glycine (2 M; Fisher BP381) Glycogen (Roche) H2 O, nuclease-free Immune complex wash buffers (high-salt and low-salt) Prepare the high-salt and low-salt versions of this buffer
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