P2-15D赛默飞配件（样品梳）Thermo Fisher

基于PCR技术的染色质沉淀分析

% APS. Pour the gel immediately into the vertical electrophoresis tank. Insert the shark's-tooth comb, and clamp it on all sides. Lay the gel flat, and let the matrix polymerize for at least 30 minutes. After polymerization, place the glass plates

Loss of Heterozygosity

with a pipette. Insert the teeth of the comb approximately 1 mm into the gel. Pre-heat the gel at 1700 volts for 15-20 minutes. F: Gel Loading Remove the samples from the freezer. Denature the samples in a thermal cycler at 95°C for 5 mins. Remove

Preparation of Polyacrylamide Gels

. DO NOT overfill. Tap any slow-running spots to quicken flow and avoid bubble formation. 10. When acrylamide solution fills the space between the plates, remove the 25 mL pipet and lay the plates flat. Insert comb teeth up and clamp. Layer excess acrylamide