30105赛默飞摇床附件Thermo Fisher 13X1

SDS-PAGE

) protein, load only 0.0125 OD ml. For Drosophila tissue culture cells, suspend 3X106 cells per 90 µl cracking buffer, and load about 20 µl per lane (0.8 mm thick gel with the BRL gel box and 20 well comb). 10. Running. Use a constant Amps power supply

Southern Blot Analysis(from Baker lab, university of Florida

of Yeastchromosomal DNA per lane to be run. Digest 0.05 to 0.01μg of plasmid DNA(a massive overkill).2.Allow restriction digest to proceed at 37°C overnight. Electrophoresis 1.Seal the gel casting platform at both ends with tape. Prepare and pour a100mL

Southern Blot Analysis(from Baker lab, university of Florida)

of plasmid DNA(a massive overkill).2.Allow restriction digest to proceed at 37℃ overnight.Electrophoresis1.Seal the gel casting platform at both ends with tape.Prepare and pour a100ml 0.8% agarose in 1X TBE.Insert the comb making sure there are no airbubbles