- ￥1755 - 10998
- Biorbyt
- 英国
- orb1516748
- 2026年08月06日
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- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
6 months from date of receipt.
- 英文名：
Recombinant mouse EMP-1 protein, GST & His
- 库存：
99
- 供应商：
Biorbyt
- 规格：
20 ug/100 ug/500 ug
| 规格： | 20 ug | 产品价格： | ￥1755.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥4680.0 |
| 规格： | 500 ug | 产品价格： | ￥10998.0 |
描述: Recombinant mouse EMP-1 protein, GST & His, with a concentration of >0.5 mg/ml, expressed in E.coli, with a molecular weight of 32 kDa, and a purity of >95% as determined by SDS-PAGE. It was purified by AC, and has a GST & His tag.
别名: CL-20; EMP 1; EMP1; EMP1_HUMAN; Epithelial membrane protein 1; Protein B4B; TMP; Tumor-associated membrane protein; Recombinant mouse EMP-1 protein, GST & His
浓度: >0.5 mg/ml
形态: Lyophilized or Liquid
靶点: Epithelial membrane protein 1
生物来源: Mouse
内毒素: Not analyzed
表达系统: E.coli
分子量: 32 kDa
防腐剂: 4M Urea (pH9.0).
蛋白序列: 16-64/160
纯度: >95% as determined by SDS-PAGE
纯化方法: AC
复溶/重构: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
标签: GST & His
保存条件: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
备注: For research use only.
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文献和实验Thrombin Cleavage of GST-Fusion protein
to cleave 1 mg of fusion protein at room temperature overnight. 1 X PBS buffer: 140mM NaCl; 2.7mM KCL; 10mM Na2HPO4; 1.8mM KH2PO4; pH 7.3 Thrombin Cleavage of Free Eluted Fusion protein First you have to calibrate amount of thrombin, temperature
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
: Protein Interactions, Second Edition Edited by Erica A. Golemis and Peter D. Adams ABSTRACT Glutathione-S-Transferase (GST) fusion proteins have a wide range of applications. This protocol is designed for IPTG-inducible
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
contains the fusion protein. Run the samples from the eluates containing protein on an SDS-polyacrylamide gel and stain with Coomassie blue dye. The GST moiety is 26 kD; therefore, add 26 kD to the predicted molecular weight of your fusion protein
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