- ￥5590
- biorbyt
- 英国
- orb1477466
- 2025年12月17日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期：
6个月
- 英文名：
Lymphocytic choriomeningitis virus Nucleoprotein Protein
- 库存：
详询客服
- 供应商：
biorbyt
- 规格：
20 ug
产品别名：(Nucleocapsid protein)(Protein N)
应用笔记：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
纯度：Greater than 85% as determined by SDS-PAGE.
生物来源：Lymphocytic choriomeningitis virus (strain WE) (LCMV)
蛋白序列：MSLSKEVKSFQWTQALRRELQGFTSDVKAAVIKDATSLLNGLDFSEVSNVQRIMRKERRDDKDLQRLRSLNQTVHSLVDPKSTSKKNVLKVGRLSAEELMTLAADLEKLKAKIMRTERPQASGVYMGNLTAQQLDQRSQILQMVGMRRPQQGASGVVRVWDVKDSSLLNNQFGTMPSLTMACMAKQSQTPLNDVVQALTDLGLLYTVKYPNLSDLERLKDKHPVLGVITEQQSSINISGYNFSLGAAVKAGAALLHGGNMLESILIKPSNSEDLLKAVLGAKKKLNMFVSDQVGDRNPYENILYKVCLSGEGWPYIACRTSVVGRAWENTTIDLTNE
分子量：44.8 kDa
表达区域：1-337aa
标签：N-terminal 10xHis-tagged and C-terminal Myc-tagged
防腐剂：If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源：E.coli
形态：Liquid or Lyophilized powder
Uniprot ID：P07400
Note：For research use only.
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文献和实验Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I and II Tetramers
-specific CD8+ and CD4+ T cell responses. Here, we describe a protocol for MHC class I and II tetramer staining of mouse T cells isolated from various tissues of mice infected with lymphocytic choriomeningitis virus (LCMV) or with murine cytomegalovirus
stomatitis virus (14 ) and lymphocytic choriomeningitis virus (15 ). HSP preparations from cells transfected with model antigens (Ags) such as ovalbumin and β-galactosidase have been shown to elicit Ag-specific cytotoxic T-lymphocytes (CTL)s
Utilizing a Retroviral RNAi System to Investigate In Vivo mTOR Functions in T Cells
, and have found that mTOR regulates memory CD8 T-cell differentiation. Here, we provide a detailed protocol for knocking down mTOR and its related molecules (raptor and FKBP12) in antigen-specific CD8 T cells. In our protocol, a mouse model of lymphocytic choriomeningitis
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