- ￥6110
- biorbyt
- 英国
- orb1477927
- 2025年12月17日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期：
6个月
- 英文名：
mazF Protein
- 库存：
详询客服
- 供应商：
biorbyt
- 规格：
20 ug
产品别名：(Toxin MazF)(mRNA interferase MazF)
应用笔记：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
纯度：Greater than 90% as determined by SDS-PAGE.
生物来源：Escherichia coli (strain K12)
蛋白序列：MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLIKAKINVLIG
分子量：13.6 kDa
表达区域：1-111aa
标签：C-terminal 6xHis-tagged
防腐剂：If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源：Yeast
形态：Liquid or Lyophilized powder
Uniprot ID：P0AE70
Note：For research use only.
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文献和实验RNA 甲基化 m6A 是如今的研究热点之一，目前主要的研究思路包括差异表达的 write，reader 和 eraser 基因分析；m6A 抗体检测全转录组甲基化水平；分析 m6A甲基化水平变化的靶基因和下游机制研究。而在 7 月 25 日的 Cell 上新发表了一篇介绍不使用 m6A 抗体的检测 mRNA m6A 水平的 Resource 文章，小编第一时间和大家分享一下。作者开发这一方法的前提是有研究报道了 MazF 能特异性的识别无修饰的 ACA 序列并发挥 RNA 酶活性在 ACA
Use of E. coli for the Production of a Single Protein
only the target protein. In the SPP system, all E. coli cellular mRNAs are eliminated by the induction of MazF, an ACA-specific mRNA interferase, which results in complete cell growth arrest. However, the mRNA for a target protein was engineered to be devoid
A Simple Method for Introducing Marker-Free Deletions in the Bacillus subtilis Genome
with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counterselection tool.
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