- ￥20150
- biorbyt
- 英国
- orb1476790
- 2025年12月17日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期：
6个月
- 英文名：
Bovine RHO Protein
- 库存：
详询客服
- 供应商：
biorbyt
- 规格：
20 ug
应用笔记：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
纯度：Greater than 85% as determined by SDS-PAGE.
生物来源：Bos taurus (Bovine)
蛋白序列：MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIMLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNNESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAVYNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQVAPA
分子量：45.0 kDa
表达区域：1-348aa
标签：N-terminal 6xHis-tagged
防腐剂：If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源：in vitro E.coli expression system
形态：Liquid or Lyophilized powder
Uniprot ID：P02699
Note：For research use only.
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文献和实验总蛋白（包括质膜和细胞器膜蛋白），但费时费力。目前，有一种最新型有效的膜蛋白提取工具，并具有高纯度、高特异性、高载量温和提取的优点。是基于1980年科学家在牛眼视网膜中发现的一种命名为Rho1D4的氨基酸序列以及其对应的抗体来作为亲和标签纯化膜蛋白取得了非常好的效果，将Rho1D4抗体链接在琼脂糖或磁珠上，用于亲和层析带有Rho1D4标签的膜蛋白，能近乎完美的解决这些研究难题。1） 什么是Rho1D4?Rho1D4是指在牛眼视网膜细胞内的牛视紫红质C端的最后九位氨基酸。Rho1D4得名于与该序列
V294.PART II，Chapter 3 划痕实验(Wound-Healing Assay)
3.). 2. Materials 1. 60-mm or other size tissue culture dishes. 2. Razor or extra fine Sharpie? marker. 3. 1 mg/mL fibronectin stock. 4. 1 mg/mL poly-L-lysine (PLL) stock. 5. Phosphate-buffered saline (PBS). 6. 2 mg/mL Bovine serum albumin (BSA
V294.PART II，Chapter 2 Boyden法体外趋化试验
of an inhibitor that is specificfor an intracellular signaling molecule or a functional blocking antibody that isspecific to a cell surface protein on cell motility by adding it to the upper chamber,in which cells are loaded. Actually, this type of experiment
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