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Insulin-like growth factor 1 (IGF-1) is also known as somatomedin C, IGF1A, IGFI, sulfation factor, and is a hormone similar in molecular structure to insulin. IGF-1 Protein binds to cell surface receptor IGF type 1 receptor, which abbreviated as "IGF1R" and modulates growth in many tissues, such as nervous tissue, lymphoid tissue, reproductive tissue, smooth muscle, endothelium, and bone. IGF-1 Protein also mediates neuroprotective mechanism. It is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons.
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文献和实验Assays for GH, IGF-I, and IGF Binding Protein-3
The growth hormone/insulin-like growth factor (GH/IGF) axis is widely investigated in disorders of growth, development, and anterior pituitary function. Methods are described for three hormones in this axis (growth hormone, IGF-I, and IGF
Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Isolation and Characterization of Calpain Activator Protein from Bovine Brain
Although in recent years the molecular structure and function of calpains have been extensively investigated, some aspects of the roles and properties of these proteinases remain obscure, particularly their precise function in the cell
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