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Human TNF Protein蛋白,orb594841,

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  • ¥2080
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  • 英国
  • orb594841
  • 2025年12月17日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 保质期

      6个月

    • 英文名

      Human TNF protein

    • 库存

      99

    • 供应商

      biorbyt

    • 规格

      10 ug

    产品描述:Recombinant Human Tumor necrosis factor(TNF),partial (Active)

    产品别名:Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2

    应用笔记:Partial

    纯度:Greater than 95% as determined by SDS-PAGE.

    生物来源:Homo sapiens (Human)

    蛋白序列:VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

    分子量:18.5 kDa

    生物活性:The ED50 as determined in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D is 10-40 pg/ml.

    表达区域:77-233aa

    内毒素:Less than 1.0 EU/µg as determined by LAL method.

    标签:C-terminal 6xHis-tagged

    防腐剂:Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.4

    来源:E.coli

    形态:Lyophilized powder

    Uniprot ID:P01375

    Note:For research use only.

    产品细节图片1
    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.









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    图标文献和实验
    相关实验
    • Production and Characterization of Recombinant Human and Murine TNF

      takes about 2 wk. Human and murine TNF can be purified from crude extracts with high yields (>50 mg from 1 L of fermentation) by hydrophobic-interaction chromatography, ion-exchange chromatography, and gel-filtration chromatography. The purity

    • Detection of TNF and TNF Receptor mRNA in Cells and Tissues

      We describe a semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique for the quantification of the messenger RNA of human and murine tumor necrosis factor α (TNFα) and related receptors. This protocol can be adapted

    • TNF in Experimental Diseases of the CNS

      Tumor necrosis factor α (TNFα) is a crucial mediator involved in the communications between immune and nervous systems in physiological conditions, and its relevance is amplified during disease. Considered originally detrimental and a target

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