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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
6个月
- 英文名:
Yeast mtb12 protein
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
20 ug
产品别名:mtb12
应用笔记:This is His-tag protein
纯度:Greater than 90% as determined by SDS-PAGE.
生物来源:Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
蛋白序列:DPASAPDVPTAAQLTSLLNSLADPNVSFANKGSLVEGGIGGTEARIADHKLKKAAEHGDLPLSFSVTNIQPAAAGSATADVSVSGPKLSSPVTQNVTFVNQGGWMLSRASAMELLQAAGN
分子量:14.1 kDa
表达区域:49-168aa
标签:N-terminal 6xHis-tagged
防腐剂:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源:Yeast
形态:Liquid or Lyophilized powder
Uniprot ID:P9WIN6
Note:For research use only.

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文献和实验转化 a. 每个转化需要50μl 细胞,5μl刚煮过的carrier DNA, 以及100ng质粒,混匀后加300μl PEG/LiAc。 b. 水浴30℃30分钟。 c. 水浴热激42℃15分钟。 d. 室温高速离心弃上清,然后用500μl ddH2O冲悬图板。 大剂量(library) 1. 制备特定感受态 a. 用小剂量的方法先将感兴趣的蛋白转到感受态中,然后再用该菌株制备
Yeast Miniprep from Robzyk and Kassir, Nucleic Acid Res. (1992) 20:3790 Materials : • 10 ml overnight culture yeast in drop out media (must be very dense) • STET (8% sucrose, 50 mM Tris pH 8, 50 mM EDTA, 5% Triton X
Protocol grow up yeast culture to appropriate density (near saturation) spin 1.5 mls of culture for 1 min in microfuge and aspirate off supernatant resuspend pellet in 200 ul breaking buffer wear gloves and add
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