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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 保质期:
6个月
- 英文名:
N-terminal 6xHis-SUMO-tagged
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
1 mg
应用笔记:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
纯度:Greater than 95% as determined by SDS-PAGE.
蛋白序列:MMAHHHHHHMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGGGSEFRT
分子量:13.1 kDa
标签:N-terminal 6xHis-SUMO-tagged
防腐剂:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源:E.coli
形态:Liquid or Lyophilized powder
Note:For research use only.

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文献和实验In order to characterize the P-type ATPase from Synechocystis 6803 [Geisler (1993) et al. J. Mol. Biol. 234 , 1284] and to facilitate its purification, we expressed an N-terminal 6xHis-tagged version of the ATPase in an ATPase deficient E. coli
Studies of the Ubiquitin Proteasome System
(see recipe ) Liquid N 2 1 M imidazole, pH 7.5 (adjusted with HCl) Ni‐NTA beads (Qiagen) Wash buffer 1 for His 6 ‐tagged proteins (see recipe
Strategies to Optimize Protein Expression in E. coli
, H., Dahlgren, L.G., Flores, A., Hammarstroem, M., Johansson, I., Kotenyova, T., Nilsson, M., Nordlund, P., and Weigelt, J. 2008b. The use of systematic N‐ and C‐terminal deletions to promote production and structural studies of recombinant proteins
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