- 询价
- BIO-RAD
- MCA463SBUV575
- 2025年12月11日
- F
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to StarBright UltraViolet 575 - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
StarBright UltraViolet 575
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
UCHT1
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名：
CD3 antibody | UCHT1
- 规格：
100 Tests/0.5ml
Mouse anti Human CD3 antibody, clone UCHT1 recognizes the human T-cell surface glycoprotein CD3 epsilon chain, also known as T-cell surface antigen T3/Leu-4 epsilon chain or CD3ε. CD3ε is a 207 amino acid, ~21kDa single pass type 1 transmembrane protein containing a single Ig-like and a single ITAM domain. Mouse anti Human CD3 antibody, clone UCHT1 was originally described as only binding to CD3ε when complexed with either the CD3δ or CD3γ subunits, as indicated by co-transfection immunofluorescence on COS cells (Salmerón et al. 1991). Mouse anti Human CD3 antibody, clone UCHT1 binds to a region in the ectodomain of human CD3ε and binds to a discontinuous epitope near an acidic region of CD3ε opposite the dimer interface; as shown by crystallography of the CD3ε/δ dimer complexed with a single chain UCHT1 antibody fragment (Arnett et al. 2004).
CD3 is expressed by all T lymphocytes and is seen in all lymphoid organs including lymph nodes and spleen. It is involved in thymocyte differentiation (Brodeur et al. 2009). Deficiency of the CD3ε chain contributes to blocking T-cell development and presentation of a severe combined immunodeficiency phenotype (Fischer et al. 2005).
Mouse anti Human CD3 antibody, clone UCHT1 has been used successfully for the activation of human peripheral blood lymphocytes by cross linking and subsequently for CD3ε surface expression by flow cytometry (Hirsh and Cohen 2006).
CD3 is expressed by all T lymphocytes and is seen in all lymphoid organs including lymph nodes and spleen. It is involved in thymocyte differentiation (Brodeur et al. 2009). Deficiency of the CD3ε chain contributes to blocking T-cell development and presentation of a severe combined immunodeficiency phenotype (Fischer et al. 2005).
Mouse anti Human CD3 antibody, clone UCHT1 has been used successfully for the activation of human peripheral blood lymphocytes by cross linking and subsequently for CD3ε surface expression by flow cytometry (Hirsh and Cohen 2006).
储存信息
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验该产品被引用文献
References for CD3 antibody
- Beverley, P.C. & Callard, R.E. (1981) Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.
Eur J Immunol. 11 (4): 329-34. - Clevers, H. et al. (1988) The transmembrane orientation of the epsilon chain of the TcR/CD3 complex.
Eur J Immunol. 18 (5): 705-10. - Salmerón, A. et al. (1991) A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.
J Immunol. 147: 3047-52. - Hirsh, M.I. and Cohen, V. (2006) Chloroquine prevents T lymphocyte suppression induced by anthrax lethal toxin.
J Infect Dis. 194: 1003-7. - Mahon, N.G. et al. (2002) Immunohistologic evidence of myocardial disease in apparently healthy relatives of patients with dilated cardiomyopathy.
J Am Coll Cardiol. 39: 455-62. - Lawson, C.A. et al. (2006) Early rheumatoid arthritis is associated with a deficit in the CD4+CD25high regulatory T cell population in peripheral blood.
Rheumatology (Oxford). 45: 1210-7. - Battaglia, A. et al. (2003) Lymphocyte populations in human lymph nodes. Alterations in CD4+ CD25+ T regulatory cell phenotype and T-cell receptor Vbeta repertoire.
Immunology. 110: 304-12. - McIntosh, R.S. et al. (1997) Analysis of the T cell receptor V alpha repertoire in Hashimoto's thyroiditis: evidence for the restricted accumulation of CD8+ T cells in the absence of CD4+ T cell restriction.
J Clin Endocrinol Metab. 82: 1140-6.
Further Reading
- Clevers, H. et al. (1988) The T cell receptor/CD3 complex: a dynamic protein ensemble.
Annu Rev Immunol. 6: 629-62. - Arnett, K.L. et al. (2004) Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.
Proc Natl Acad Sci U S A. 101: 16268-73.
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Mouse anti Human CD3:StarBright UltraViolet 575，clone UCHT1
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