小鼠抗人 CD38 抗体:StarBright Red 715 荧光标记
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小鼠抗人 CD38 抗体:StarBright Red 71

5 荧光标记
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  • 询价
  • BIO-RAD已认证
  • MCA1019SBR715
  • 2025年12月20日
  • F
  • Human
  • 详细信息
  • 文献和实验
  • 技术资料
  • 免疫原

    Namalwa human B-cell line.

  • 亚型

    IgG1

  • 形态

    Purified IgG conjugated to StarBright Red 715 - liquid

  • 克隆性

    Monoclonal Antibody

  • 标记物

    StarBright Red 715

  • 适应物种

    Human

  • 保质期

    自发货之日起 12 个月

  • 目录编号

    AT13/5

  • 供应商

    伯乐生命医学产品(上海)有限公司

  • 应用范围

    F

  • 浓度

    For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.

  • 抗体名

    CD38 antibody | AT13/5

  • 规格

    100 Tests/0.5ml

小鼠抗人CD38抗体,克隆AT13/5 可识别CD38,该蛋白亦称为ADP-核糖基环化酶/环状ADP-核糖水解酶1、2'-磷酸-ADP-核糖基环化酶、ADP-核糖基环化酶1、环状ADP-核糖水解酶1或T10。CD38是一种由300个氨基酸组成的约45 kDa单次跨膜II型糖蛋白,克隆AT13/5的反应模式与CD38抗体簇的其他抗体一致。CD38在浆细胞、单核细胞、早期淋巴细胞及活化的T细胞中表达。

CD38广泛应用于B细胞和T细胞分化与活化过程的研究。该蛋白是一种多功能酶,参与钙信号传导和烟酰胺腺嘌呤二核苷酸(NAD⁺)代谢过程(de Oliveira等,2018)。

 

项目 详情
靶物种
产品形式 纯化IgG,与StarBright Red 715偶联 - 液体
制备方法 通过Protein A亲和层析从组织培养上清液中纯化IgG
缓冲溶液 磷酸盐缓冲盐水
防腐剂/稳定剂 0.09% (NaN₃)、1%牛血清白蛋白、0.1% Pluronic F68、0.1% PEG 3350、0.05% Tween 20
免疫原 Namalwa人B细胞系
近似蛋白浓度 关于StarBright染料偶联试剂的浓度信息,请访问我们的FAQ页面
融合伙伴 来自免疫BALB/c小鼠的脾细胞与小鼠NS1骨髓瘤细胞系细胞融合
最大激发/发射波长 荧光染料:StarBright Red 715
激发最大值 (nm):638
发射最大值 (nm):712
用途说明 仅用于研究用途
有效期 自发货之日起12个月
专利信息 本产品受美国专利第10,150,841号及相关美国和外国对应专利保护

储存信息

本产品在环境温度下运输。
请于+4°C条件下储存。切勿冷冻。
本品应保持原液状态储存。


应用

据报道,本产品可用于以下实验体系。该信息源自我们实验室的内部测试、同行评审的出版物或研发人员的直接交流。更多细节请参阅引用的参考文献。常规实验方案建议请访问抗体实验方案专页。

应用名称 已验证 最小稀释度 最大稀释度
流式细胞术 原液  

若本产品未针对特定技术进行测试,并不代表其不可用于该技术。建议的工作稀释度仅供参考。建议使用者根据自身实验体系,使用适当的阴性/阳性对照进行产品滴定。

流式细胞术

取5µl建议工作稀释液,用于标记100µl体系中10⁶个细胞。最佳实践建议在加样前以6,000g离心5分钟。

 

Mouse anti Human CD38 antibody, clone AT13/5 recognizes CD38, also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1 or T10. CD38 is a 300 amino acid ~45 kDa single pass type II transmembrane glycoprotein and Mouse anti Human CD38 antibody, clone AT13/5 has a reactivity pattern consistent with other antibodies of the CD38 cluster. CD38 is expressed by plasma cells, monocytes, early lymphoid cells and activated T cells.

CD38 is widely used to study the processes of B and T differentiation and activation. CD38 is a multifunctional enzyme involved in calcium signaling and nicotinamide adenine dinucleotide (NAD+) metabolism (de Oliveira et al. 2018).

储存信息

This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

应用

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat  
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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图标文献和实验
该产品被引用文献

Source Reference

  1. Ellis, J.H. et al. (1995) Engineered anti-CD38 monoclonal antibodies for immunotherapy of multiple myeloma.
    J Immunol. 155 (2): 925-37.

References for CD38 antibody

  1. Corcione, A. et al. (2009) CX3CR1 is expressed by human B lymphocytes and mediates [corrected] CX3CL1 driven chemotaxis of tonsil centrocytes.
    PLoS One. 4: e8485.
  2. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
    BMC Immunol. 12: 2.
  3. Tipping, A.J. et al. (2009) High GATA-2 expression inhibits human hematopoietic stem and progenitor cell function by effects on cell cycle
    Blood. 113: 2661-72.
  4. Slyker, J.A. et al. (2012) Acute cytomegalovirus infection is associated with increased frequencies of activated and apoptosis-vulnerable T cells in HIV-1-infected infants.
    J Virol. 86 (20): 11373-9.
  5. Slyker, J.A. et al. (2012) The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
    Front Immunol. 3: 399.
  6. Kelleher, M. et al. (2011) Comparative Kinetics of Immune Responses and Changes in Cellular Sub-Sets Detected in Colorectal Cancer Patients Vaccinated with MVA-5T4 (TroVax) Administered Alongside Two Different Chemotherapy Regimens
    J Cancer Therapy. 02 (01): 54-64.

Further Reading

  1. Malavasi, F. et al. (1994) Human CD38: a glycoprotein in search of a function.
    Immunol Today. 15 (3): 95-7.
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小鼠抗人 CD38 抗体:StarBright Red 715 荧光标记
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