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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
B6.1 CTL cell line.
- 亚型:
IgG1
- 形态:
Purified IgG conjugated to StarBright UltraViolet 510 - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
StarBright UltraViolet 510
- 适应物种:
Mouse
- 保质期:
自发货之日起 12 个月
- 目录编号:
PC61.5.3
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F
- 浓度:
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名:
CD25 antibody | PC61.5.3
- 规格:
100 Tests/0.5ml
Rat anti Mouse CD25 antibody, clone PC61.5.3 reacts with the low affinity alpha chain of the interleukin-2 receptor present on activated T and B cells in mice. Rat anti Mouse CD25 antibody, clone PC61.5.3 is reported to inhibit IL-2 binding and IL-2 dependent proliferation.
储存信息
This product is shipped at ambient temperature.
Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验该产品被引用文献
References for CD25 antibody
- Ceredig, R. et al. (1985) Expression of interleukin-2 receptor as a differentiation marker on intrathymic stem cells.
Nature 314: 98-100. - Lowenthal, J.W. et al. (1985) High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61.
J Immunol. 135 (6): 3988-94. - Hashimoto, N. et al. (1986) Dissociation of interleukin 2-dependent and -independent B cell proliferation with monoclonal anti-interleukin 2 receptor antibody.
Eur J Immunol. 16 (3): 317-20. - Moreau, J.L. et al. (1987) Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site.
Eur J Immunol. 17 (7): 929-35. - Yaqoob, P. & Calder, P.C. (1997) Glutamine requirement of proliferating T lymphocytes.
Nutrition. 13 (7-8): 646-51. - Scotland, R.S. et al. (2011) Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice.
Blood. 118 (22): 5918-27. - Karali, D. et al. (2016) T cell regulation by Phlomis lanata protein extracts in mice.
Pharm Biol. 54 (2): 207-14. - Szuster-Ciesielska, A. et al. (2019) Immunogenic Evaluation of Ribosomal P-Protein Antigen P0, P1, and P2 and Pentameric Protein Complex P0-(P1-P2)2 of Plasmodium falciparum in a Mouse Model.
J Immunol Res. 2019: 9264217.
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Rat anti Mouse CD25:StarBright UltraViolet 510
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