- 询价
- BIO-RAD
- MCA2307F
- 2025年10月17日
- F, IP, WB
- Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
ConA/PMA activated porcine peripheral blood cells.
- 亚型：
IgG2a
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Pig
- 保质期：
自发货之日起 12 个月
- 目录编号：
1H6/8
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F, IP, WB
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD5 antibody | 1H6/8
- 规格：
0.1 mg
Mouse anti Pig CD5 antibody, clone 1H6/8 recognizes pig CD5. Clone 1H6/8 was clustered as CD5a at the Second International Swine CD antigen Workshop (Saalmuller et al. 1998), based on its cellular distribution, ability to completely inhibit the binding of monoclonal antibody clone b53b7, the internal control for the second workshop and the immunoprecipitation of the same 55 kDa + 60 kDa doublet as the control antibody (Pescowitz et al. 1998). Porcine CD5 is expressed by all circulating peripheral blood T lymphocytes and some B cells. CD5 is not expressed by porcine γ/δ T cells (Saalmuller et al. 1994) and can hence be used for the discrimination between NK cells (CD4- CD8+ CD5-) and MHC-restricted cytotoxic T cells (CD4- CD8+ CD5+).
No reactivity of Mouse anti Pig CD5 antibody, clone 1H6/8 was noted with canine, bovine, equine or human tissue samples (Doménech et al. 2003).
No reactivity of Mouse anti Pig CD5 antibody, clone 1H6/8 was noted with canine, bovine, equine or human tissue samples (Doménech et al. 2003).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat | 1/10 |
| Immunoprecipitation | ✓ | ||
| Western Blotting | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to 1x106 cells in 100μl
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文献和实验该产品被引用文献
References for CD5 antibody
- Pescovitz, M.D. et al. (1998) Analyses of monoclonal antibodies reacting with porcine CD5: results from the Second International Swine CD Workshop.
Vet Immunol Immunopathol. 60 (3-4): 269-73. - Saalmüller, A. et al. (1998) Overview of the Second International Workshop to define swine cluster of differentiation (CD) antigens.
Vet Immunol Immunopathol. 60 (3-4): 207-28. - Doménech, N. et al. (2003) A new epitope on swine CD5 molecule detected by monoclonal antibody 5F12/9.
Hybrid Hybridomics. 22 (3): 179-82. - Sánchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
Arch Virol. 148 (12): 2307-23. - Thierry, A. et al. (2012) Identification of invariant natural killer T cells in porcine peripheral blood.
Vet Immunol Immunopathol. 149 (3-4): 272-9. - Skovdal, S.M. et al. (2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.
PLoS One. 14 (10): e0223647. - Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
PLoS One. 16 (5): e0249366. - Jarosz, Ł. et al. (2021) The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators of the Specific and Nonspecific Immune Response.
Probiotics Antimicrob Proteins. Oct 01 [Epub ahead of print].
Further Reading
- Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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Mouse anti Pig CD5:FITC，clone 1H6/8
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