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Cytokeratin 10 Antibody, anti-

human, FITC, REAdye_lease™, 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-126-433
  • 2025年12月09日
  • MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • cell line
  • human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Cytokeratin 10

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REAL611

    • 标记物

      FITC

    • 适应物种

      human

    • 保质期

      24个月

    • 供应商

      Miltenyi biotec

    • 宿主

      cell line

    • 应用范围

      MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 抗体英文名

      Cytokeratin 10 Antibody, anti-human, REAdye_lease™

    • 抗体名

      Cytokeratin 10 Antibody, anti-human, REAdye_lease™

    • 规格

      200 µL

    Detection of Cytokeratin 10+ cellsClone REAL611 is an antibody fragment derived from the full Cytokeratin 10 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity. | Clone REAL611 recognizes the human Cytokeratin 10, which belongs to the type I (acidic) cytokeratin family. Cytokeratins, which are found in almost all epithelial cells, are polypeptides with keratin-containing intermediate filaments, which ensure form stability and cohesiveness. Cytokeratin 10 is located in all suprabasal cell layers including stratum corneum and is associated with keratin-1. | For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

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    图标文献和实验
    该产品被引用文献
    Moll, R. et al. (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31 (1): 11–24. | Arin, M. J. et al. (1999) A novel substitution in keratin 10 in epidermolytic hyperkeratosis. J. Invest. Dermatol. 112 (4): 506–508. | Schweizer, J. et al. (2006) New consensus nomenclature for mammalian keratins. J. Cell Biol. 174 (2): 169–174.
    相关实验
    • Anti-GFP/BrdU In Situ Immunostaining

      Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative:  4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit

    • Annexin V-FITC/PI双染细胞凋亡检测方法

      Buffer;(2)细胞收集。悬浮细胞收集:离心5分钟;贴壁细胞:用不含EDTA的胰酶消化收集后(注:胰酶消化时间不宜过长,否则会影响细胞膜上磷脂酰丝氨酸与Annexin V-FITC的结合),于室温2000rpm离心5~10分钟,收集细胞;(3)细胞洗涤:用预冷1×PBS(4℃)重悬细胞一次,2000rpm离心5~10分钟,洗涤细胞;(4)加入300μL 的1×Binding Buffer 悬浮细胞;(5)Annexin V-FITC标记:加入5μL的Annexin V-FITC混匀后,避光,室温

    • RUVKUN Antibody Staining Protocol revised 10/29/90

      hours at RT. 13. Wash for 4-24 hours at RT in Buffer B (usually do 5 quick washes and then 2-3 hours later do 1-2 more). 14. Incubate with 2 in Buffer A, 4-24 hours, RT (1:200 FITC-coupled goat anti-rabbit antibody for lin-14

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    Cytokeratin 10 Antibody, anti-human, FITC, REAdye_lease™, 200 µL
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