- 询价
- Miltenyi Biotec
- 德国
- 130-123-370
- 2025年12月09日
- MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- cell line
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Actin (Smooth Muscle)
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，4-8℃
- 克隆性：
REAL650
- 标记物：
PE
- 适应物种：
human, mouse
- 保质期：
24个月
- 供应商：
Miltenyi biotec
- 宿主：
cell line
- 应用范围：
MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 抗体英文名：
Actin (Smooth Muscle) Antibody, anti-human, REAdye_lease™
- 抗体名：
Actin (Smooth Muscle) Antibody, anti-human, REAdye_lease™
- 规格：
200 µL
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文献和实验LABELING MUSCLE ACTIN WITH IATR
. 4.Collect actin from dialysis tubing,add 100 mM KCl and 2 mM MgCl2 to induce polymerization.Let sit on ice for 20 min. 5.Get >=1 mg IATR in a test tube.Add 100-200 µl acetone and make a fine slurry by grinding particles against the tube with a pipet. 6.Add
Labeling Muscle Actin with 5-iodoacetamidofluorescein
1 overnight. 4.Collect actin from dialysis tubing,add 100 mM KCl and 2 mM MgCl2 to induce polymerization.Let sit on ice for 20 min. 5.Get 2.4 mg IAF in a test tube.Add 100-200 µl acetone and make a fine slurry by grinding particles against the tube
by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
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