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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD41/CD61
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,4-8℃
- 克隆性:
REAL179
- 标记物:
APC
- 适应物种:
human
- 保质期:
24个月
- 供应商:
Miltenyi biotec
- 宿主:
cell line
- 应用范围:
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度:
1:50
- 抗体英文名:
CD41/CD61 Antibody, anti-human, REAlease®
- 抗体名:
CD41/CD61 Antibody, anti-human, REAlease®
- 规格:
100 tests in 200 µL
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文献和实验30s,接着40个循环的94℃ 15 s,65℃ 20 s,68℃ 5 min],其中使用了1单位的ELONGASE酶混合物[PCR 50ml终体积,其中60 mM Tris-SO4(pH9.1),18 mM (NH4)2SO4,2 mM MgSO4,dNTP每个200 µM,引物每个400 nM ],其中分别是AAP和引物TSC-2-2826(A带)或引物TSC-2-1732(B带)一起使用。每个PCR产物(带1和带3)取10µl 在0.8%琼脂糖上,1倍TAE凝胶电泳和SYBRò绿染色(分子
MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
浓度为 1x107cells/ 100 μl,从中取 1 - 2x106 cells; 2. 用去离子水将 10xBuffer IS 稀释为 1x,准备 3 ml 稀释后的 Buffer IS; 3.取 1ul Strep-Jactin®PE 或 Strep-Tactin®APC,0.8μl MHC I-Strep,与 1x Buffer IS 混匀为 10ul 的终体积,冰上孵育 45 分钟; 4.将上一步孵育完的 Streptamers 复合物加入到细胞悬液中轻轻吹打混匀,冰上孵育 45 分钟
In a 2 ml round-bottomed microcentrifuge tube mix 100 μl anti-coagulated blood with 1 ml pre-diluted Red Cell Lysis Buffer (diluted 1:5 from the original concentration supplied, stored at 2-8°C and used cold). If less than 10 μl of blood
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