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CD10 Antibody, anti-human, APC

, REAlease®, 100 tests in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-119-590
  • 2025年12月09日
  • Flow cytometry
  • cell line
  • human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD10

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REAL318

    • 标记物

      APC

    • 适应物种

      human

    • 保质期

      24个月

    • 供应商

      Miltenyi biotec

    • 宿主

      cell line

    • 应用范围

      Flow cytometry

    • 浓度

      1:50

    • 抗体英文名

      CD10 Antibody, anti-human, REAlease®

    • 抗体名

      CD10 Antibody, anti-human, REAlease®

    • 规格

      100 tests in 200 µL

    Identification and enumeration of CD10+ cells by flow cytometryClone REAL318 is an antibody fragment derived from the full CD10 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity. | Clone REAL318 recognizes the human CD10 antigen, also known as the common acute lymphoblastic leukemia antigen (CALLA). CD10 is a 100 kDa cell surface molecule identical to human membrane-associated neutral endopeptidase (NEP) and also known as neprilysin or enkephalinase. | Human CD10 is expressed on a wide variety of normal and neoplastic cell types from different tissues including neural and hematopoietic cells. | It is expressed on pre– and pro–B cells and is involved in B cell development and differentiation. The antigen is also present on mature neutrophils, T cell precursors, and some T cell leukemias/lymphomas. Furthermore, CD10 is expressed on neoplastic cells of several B lymphoid leukemias/lymphomas. | The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

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    图标文献和实验
    相关实验
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      30s,接着40个循环的94℃ 15 s,65℃ 20 s,68℃ 5 min],其中使用了1单位的ELONGASE酶混合物[PCR 50ml终体积,其中60 mM Tris-SO4(pH9.1),18 mM (NH4)2SO4,2 mM MgSO4,dNTP每个200 µM,引物每个400 nM ],其中分别是AAP和引物TSC-2-2826(A带)或引物TSC-2-1732(B带)一起使用。每个PCR产物(带1和带3)取10µl 在0.8%琼脂糖上,1倍TAE凝胶电泳和SYBRò绿染色(分子

    • MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞

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    • Dynabeads® DNA DIRECT Blood

             In a 2 ml round-bottomed microcentrifuge tube mix 100 μl anti-coagulated blood with 1 ml pre-diluted Red Cell Lysis Buffer (diluted 1:5 from the original concentration supplied, stored at 2-8°C and used cold). If less than 10 μl of blood

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