- 询价
- Miltenyi Biotec
- 德国
- 130-121-262
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- cell line
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD104 (Integrin β4)
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，4-8℃
- 克隆性：
REAL151
- 标记物：
VioBlue
- 适应物种：
human
- 保质期：
24个月
- 供应商：
Miltenyi biotec
- 宿主：
cell line
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度：
1:50
- 抗体英文名：
CD104 (Integrin β4) Antibody, anti-human, REAlease®
- 抗体名：
CD104 (Integrin β4) Antibody, anti-human, REAlease®
- 规格：
30 tests in 60 µL
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文献和实验MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
用量（1.6 μl MHC I-Strep ＋2 μl Strep-Tactin® PE in 20 ul buffer IS） 3 倍 Streptamers 用量（2.4 μl MHC I-Strep ＋3 μl Strep-Jactin® PE in 30 μl buffer IS） 4 倍 Streptamers 用量（3.2 μl MHC I-Strep ＋4 μl Strep-Jactin® PE in 40 μl buffer IS） Streptamers 的解离
Use a 1L bag for CD3 T cell separation. Add 100 ml of air to the bag. 3) Add 5 x 108 CD3 T cells to the 1L bag in 50-60 ml Buffer 1. 4) Add 4.0 ml (corresponding to 1.6 x 109 Dynabeads®) of washed Dynabeads® CD3⁄CD
In a 2 ml round-bottomed microcentrifuge tube mix 100 μl anti-coagulated blood with 1 ml pre-diluted Red Cell Lysis Buffer (diluted 1:5 from the original concentration supplied, stored at 2-8°C and used cold). If less than 10 μl of blood
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