CD8b Antibody, anti-human, PE-

Identification and enumeration of CD8b+ cells by flow cytometryClone REA715 recognizes the human CD8b antigen, a single-pass type I membrane protein and member of the immunoglobulin superfamily, also known as T cell surface glycoprotein Lyt-3. The CD8 glycoprotein is expressed by thymocytes, mature T cells, and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex class I antigens and in signal transduction during the course of T cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. Most of peripheral blood CD8+ T lymphocytes express the CD8a/CD8b heterodimer, while CD8+CD16+ natural killer cells and CD8+ T cell receptor γ/δ+ T lymphocytes express only the CD8a/CD8a homodimer. CD8b can therefore be used to selectively bind to CD8+ T cells while excluding CD8+ NK cells. | Additional information: Clone REA715 displays negligible binding to Fc receptors.