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CD79b Antibody, anti-human, PE

, REAfinity™, 30 tests in 60 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-123-967
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • human, non-human primate
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD79b

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REA120

    • 标记物

      PE

    • 适应物种

      human, non-human primate

    • 保质期

      24个月

    • 供应商

      Miltenyi biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:50

    • 抗体英文名

      CD79b Antibody, anti-human, REAfinity™

    • 抗体名

      CD79b Antibody, anti-human, REAfinity™

    • 规格

      30 tests in 60 µL

    Identification and enumeration of CD79b+ cells by flow cytometryClone REA120 detects the extracellular epitope of CD79b, the β chain of heterodimeric molecule CD79. CD79 is composed of an α chain (CD79a) and a β chain (CD79b) and is associated with surface immunoglobulins (Igs) to form the B cell receptor (BCR). CD79b is 37–39 kDa type I integral membrane protein and like CD79a contains an intracellular immunoreceptor tyrosine-based activation motif (ITAM). CD79a/CD79b heterodimer facilitates differentiation of pre–B cells from pro–B cells, surface expression of sIg, signal transduction following antigen recognition, and enodocytosis of recognised antigens. Patients with chronic lymphocytic leukemia (CLL) B cells lack a functional BCR which is often associated with mutations in CD79b coding region. | Additional information: Clone REA120 displays negligible binding to Fc receptors. |

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    图标文献和实验
    该产品被引用文献
    Lin, J. and Justement, L. B. (1992) The MB-1/B29 heterodimer couples the B cell antigen receptor to multiple src family protein tyrosine kinases. J Immunol 149: 1548–1555. | Koyama, M. et al. (1997) CD79α/CD79β heterodimers are expressed on pro-B cell surfaces without associated μ heavy chain. Int. Immunol. 9: 1767–1772. | Gordon, M. S. et al. (2000) Aberrant B cell receptor signaling from B29 (Igβ, CD79b) gene mutations of chronic lymphocytic leukemia B cells. Proc. Natl. Acad. Sci. U.S.A. 97: 5504–5509.
    相关实验
    • T-cell dependent antigen specific in situ staining (Pe)

      HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’.  Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide

    • Western Blotting with Horseradish Pe

      7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED IN ALL STEPS

    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC Anti-Mouse Bead Kit 1)        Completely resuspend the AbC capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

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    文献支持
    CD79b Antibody, anti-human, PE, REAfinity™, 30 tests in 60 µL
    询价