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CD79a Antibody, anti-human, PE

, REAfinity™, 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-119-722
  • 2025年12月09日
  • MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD79a

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REA1142

    • 标记物

      PE

    • 适应物种

      human

    • 保质期

      24个月

    • 供应商

      Miltenyi biotec

    • 宿主

      human cell line

    • 应用范围

      MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:50

    • 抗体英文名

      CD79a Antibody, anti-human, REAfinity™

    • 抗体名

      CD79a Antibody, anti-human, REAfinity™

    • 规格

      200 µL

    Recombinant antibody for the detection of CD79a+ cellsClone REA1142 recognizes the human CD79a antigen, which is also known as mb-1 and Ig α. Along with CD79b (Ig β), CD79a is associated with surface Ig (sIg) to form the B cell antigen receptor complex. CD79a is a 47 kDa glycoprotein and comprises a single extracellular immunoglobulin domain, a transmembrane domain, and an signaling intracellular domain with immunoreceptor tyrosine-based activation motif (ITAM). CD79a/CD79b heterodimer facilitates differentiation of pre-B cells from pro-B cells, surface expression of sIg, signal transduction following antigen recognition and endocytosis of recognized antigens. Surface expression of CD79a diminishes in plasma cells, where it is mainly found as an intracellular molecule. CD79 is considered a B cell–specific marker and is often used to identify the B cell lineage of acute lymphoblastic leukemia. Recent reports however suggest expression of CD79a in T cell acute lymphoblastic leukemia. Individuals lacking CD79a have low/absent circulating pre-B cells and serum antibodies due to a block in B cell development. | Additional information: Clone REA1142 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Mason, D. Y. et al. (1991) The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol 147 (11): 2474–2482. | Koyama, M. et al. (1997) CD79α/CD79β heterodimers are expressed on pro-B cell surfaces without associated μ heavy chain. Int. Immunol. 9: 1767–1772. | Lin, J. and Justement, L. B. (1992) The MB-1/B29 heterodimer couples the B cell antigen receptor to multiple src family protein tyrosine kinases. J Immunol 149: 1548–1555. | Minegishi, Y. et al. (1999) Mutations in Igalpha (CD79a) result in a complete block in B-cell development. J. Clin. Invest. 104 (8): 1115–1121.
    相关实验
    • T-cell dependent antigen specific in situ staining (Pe)

      -conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining

    • AbC Anti-Mouse Bead Kit

      suspension. 5)        Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)        Add a pre-titrated

    • AbC Anti-Rat/Hamster Bead Kit

      1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)     

    图标技术资料

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    文献支持
    CD79a Antibody, anti-human, PE, REAfinity™, 200 µL
    询价