- 询价
- Miltenyi Biotec
- 德国
- 130-128-460
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- human cell line
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD47
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，4-8℃
- 克隆性：
REA220
- 标记物：
Vio Bright R720
- 适应物种：
human
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度：
1:50
- 抗体英文名：
CD47 Antibody, anti-human, REAfinity™
- 抗体名：
CD47 Antibody, anti-human, REAfinity™
- 规格：
30 tests in 60 µL
Identification and enumeration of CD47+ cells by flow cytometryClone REA220 recognizes CD47, a heavily glycosylated protein with an extracellular IgV domain, five transmembrane domains, and a cytoplasmic tail, which exists in four different isoforms. CD47 interact with integrins, thrombospondin-1 (TSP-1), and cell surface gylcoproteins SIRPα and SIRPβ. Owing to these interactions, CD47 plays an important role in lymphocyte homeostasis, maturation and activation of dendritic cells, cellular transmigration, and phagocytosis of cells. Expression of CD47 is found ubiquitously. | Additional information: Clone REA220 displays negligible binding to Fc receptors. |
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文献和实验该产品被引用文献
Demeure, C. E. et al. (2000) CD47 engagement inhibits cytokine production and maturation of human dendritic cells. J Immunol 164: 2193–2199. | Burger, P. et al. (2012) CD47 functions as a molecular switch for erythrocyte phagocytosis. Blood 119: 5512–5521. | Kusakari, S. et al. (2008) Trans-endocytosis of CD47 and SHPS-1 and its role in regulation of the CD47–SHPS-1 system. J. Cell. Sci. 121: 1213–1223.
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CD47 Antibody, anti-human, Vio® Bright R720, REAfinity™, 30 tests in 60 µL
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