- 询价
- Miltenyi Biotec
- 德国
- 130-121-228
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- human cell line
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD45RB
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，4-8℃
- 克隆性：
REA1180
- 标记物：
PE-Vio 770
- 适应物种：
mouse
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度：
1:50
- 抗体英文名：
CD45RB Antibody, anti-mouse, REAfinity™
- 抗体名：
CD45RB Antibody, anti-mouse, REAfinity™
- 规格：
150 µg in 1 mL
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文献和实验with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes
. The mixture is stable at -20°C. (ii) Use a PCR purification kit (QIAGEN®). Add 500µl of binding buffer PB, and pass the mixture though a column. Wash with EtOH containing buffer PE, and elute with bidistilled water. Concentrate the probe up to 10-20µl
; 50%; PBT 30% MeOH; 70%; PBT 100% PBT For larval imaginal discs, skip the re-hydration step and start directly at the RNAseA step below, after fixation; Incubate tissues in 1ml of 100-200µg/ml RNAseA in PBT
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