CD3ε (intracellular) Antibody, anti-human/mouse, FITC, REAfinity™, 100 tests in 200 µL产品图
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CD3ε (intracellular) Antibody,

anti-human/mouse, FITC, REAfinity™, 100 tests in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-116-185
  • 2025年12月09日
  • Intracellular flow cytometry
  • human cell line
  • human, mouse
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德国美天旎生物技术有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原：

      CD3ε (intracellular)

    • 亚型：

      recombinant human IgG1

    • 形态：

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件：

      避光，4-8℃

    • 克隆性：

      REA975

    • 标记物：

      FITC

    • 适应物种：

      human, mouse

    • 保质期：

      24个月

    • 供应商：

      Miltenyi Biotec

    • 宿主：

      human cell line

    • 应用范围：

      Intracellular flow cytometry

    • 浓度：

      1:50

    • 抗体英文名：

      CD3ε (intracellular) Antibody, anti-human/mouse, REAfinity™

    • 抗体名：

      CD3ε (intracellular) Antibody, anti-human/mouse, REAfinity™

    • 规格：

      100 tests in 200 µL

    Identification and enumeration of CD3ε (intracellular)+ cells by flow cytometryClone REA975 recognizes an intracellular part of the CD3ε chain (activation epitope). CD3ε is a single-pass type I membrane protein, which is a part of the CD3 complex and a subunit of the TCR complex. The CD3 complex mediates signal transduction in several intracellular pathways and has a role in T cell activation and antigen recognition by binding the peptide/MHC antigen complex. CD3ε is expressed on all mature T lymphocytes, NKT cells, and during the development of thymocytes. | Additional information: Clone REA975 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Gold, D. P. et al. (1987) Evolutionary relationship between the T3 chains of the T-cell receptor complex and the immunoglobulin supergene family. Proc. Natl. Acad. Sci. U.S.A. 84 (21): 7649–7653. | Clevers, H. et al. (1988) Characterization and expression of the murine CD3-epsilon gene. Proc. Natl. Acad. Sci. U.S.A. 85 (22): 8623–8627. | Soudais, C. et al. (1993) Independent mutations of the human CD3-epsilon gene resulting in a T cell receptor/CD3 complex immunodeficiency. Nat. Genet. 3 (1): 77–81.
    相关实验

    • Intracellular Cytokine Staining Protocol

      28 (2ug/ml soluble) 5hrMonensinJES5-16E3IL-12mouse PECmIFNg (100ng/ml) (2hr)/LPS (100ng/ml) (22hr)2hr/22hr-MonensinC17.8GM-CSFmouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble

    • T-Cell Activation Using mAb to CD3

      splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16

    • Intracellular Immunofluorescent Staining for Flow Cytometry

      concentrations for this application is 0.5-0.06µg/106 cells, 100µl staining volume. eBioscience fluorochrome-labeled anti-cytokine antibodies are also available in 20µl/test sizes. If using these reagents, add 20µl of pre-titrated antibody to the appropriate tube

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    CD3ε (intracellular) Antibody, anti-human/mouse, FITC, REAfinity™, 100 tests in 200 µL
    询价