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CD366 (TIM-3) Antibody, anti-m

ouse, PE, REAfinity™, 150 µg in 1 mL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-118-563
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD366 (TIM-3)

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REA602

    • 标记物

      PE

    • 适应物种

      mouse

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:50

    • 抗体英文名

      CD366 (TIM-3) Antibody, anti-mouse, REAfinity™

    • 抗体名

      CD366 (TIM-3) Antibody, anti-mouse, REAfinity™

    • 规格

      150 µg in 1 mL

    Identification and enumeration of CD366 (TIM-3)+ cells by flow cytometryClone REA602 reacts with the mouse CD366 (TIM-3) antigen, which is a Tʜ1-specific cell surface protein expressed by differentiated CD4+ Tʜ1 and CD8+ Tc1 cells and macrophages. It is absent on CD4+ Tʜ2 and CD8+ Tc2 cells. CD366 (TIM-3) has been proposed to inhibit Tʜ1-mediated immune responses. CD366 (TIM-3) absence enhances the severity of experimental autoimmune encephalomyelitis in mice. The interaction of CD366 (TIM-3) and galectin-9 induces apoptosis of Tʜ1 cells. | Additional information: Clone REA602 displays negligible binding to Fc receptors. |

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    图标文献和实验
    该产品被引用文献
    Monney, L. et al. (2002) Tʜ1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease. Nature 415: 536–541. | van de Weyer, P. S. et al. (2006) A highly conserved tyrosine of Tim-3 is phosphorylated upon stimulation by its ligand galectin-9. Biochem. Biophys. Res. Commun. 351 (2): 571–576.
    相关实验
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      7211011 小鼠淋巴细胞分离液 达优 100339 Ultra-LEAF Purified anti-mouse CDAntibody BioLegend 102115 Ultra-LEAF Purified anti-mouse CD28 Antibody BioLegend   CD3/CD28 Streptamer® 多聚体法:   货号 名称

    • Th17 Polarization of Mouse CD4 Cells

      of anti-mouse CD28, clone 37.51(5μg/mL),IL-6 (50ng/mL),TGF-β1 (1ng/mL), anti-mouse IL-4 (10μg/mL), anti-mouse IFN-γ (10μg/mL), and 300 nM of FICZ. Alternatively, mouse IL-23 (5ng/ ml) can be used in place of FICZ. 3)        On day

    • Immunofluorescent Staining of Mouse and Rat Leukocytes

      16 (Mouse BD Fc Block; Cat No. 553141/553142) or anti-rat CD32 (Rat BD Fc Block; Cat. No. 550270/550271) is recommended. BD Fc Block can be added to cells (~0.25 µg per million cells, 3 - 5 min, 4°C) and need not be washed out prior to addition of primary

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    文献支持
    CD366 (TIM-3) Antibody, anti-mouse, PE, REAfinity™, 150 µg in 1 mL
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