- 询价
- Miltenyi Biotec
- 德国
- 130-116-602
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry
- human cell line
- human, non-human primate, other
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD32
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，4-8℃
- 克隆性：
REA997
- 标记物：
APC-Vio 770
- 适应物种：
human, non-human primate, other
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry
- 浓度：
1:50
- 抗体英文名：
CD32 Antibody, anti-human, REAfinity™
- 抗体名：
CD32 Antibody, anti-human, REAfinity™
- 规格：
100 tests in 200 µL
Identification and enumeration of CD32+ cells by flow cytometryClone REA997 recognizes the human CD32 antigen, also known as FcγRII. CD32 is a 40 kDa low affinity receptor for the Fc fragment of aggregated IgG. Due to alternative splicing three different isoforms of CD32 are expressed on hematopoietic cells: FcRIIA, FcRIIB, and FcRIIC. The cytoplasmic domains of FcRIIA and FcRIIC contain an activatory ITAM motif whereas FcRIIB is an inhibitory receptor containing an ITIM motif. Different isoforms of CD32 are expressed on B cells, monocytes, dendritic cells, granulocytes, and platelets. Clone REA997 recognizes all isoforms of CD32 without blocking the binding of immune complexes. | Additional information: Clone REA997 displays negligible binding to Fc receptors.
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文献和实验该产品被引用文献
Stuart, S. G. et al. (1989) Human IgG Fc receptor (hFcRII; CD32) exists as multiple isoforms in macrophages, lymphocytes and IgG-transporting placental epithelium. EMBO J. 8 (12): 3657–3666. | Van Den Herik-Oudijk, I. E. et al. (1994) Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes. J Immunol 152 (2): 574–585. | Båve, U. et al. (2003) FcγRIIa is expressed on natural IFN-α-producing cells (plasmacytoid dendritic cells) and is required for the IFN-α production induced by apoptotic cells combined with lupus IgG. J Immunol 171: 3296–3302.
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CD32 Antibody, anti-human, APC-Vio® 770, REAfinity™, 100 tests in 200 µL
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