ZAP70 Antibody, anti-human, Vi

Identification and enumeration of ZAP70+ cells by flow cytometryClone REA814 recognizes the human zeta-chain-associated protein kinase 70 (ZAP70). ZAP70 plays an essential role in regulation of the adaptive immune response. It is normally expressed near the surface membrane of T cells and natural killer cells. Zap70 associates with the ζ subunit of the T cell antigen receptor and plays a critical role in T cell–signaling. It regulates both T cell–activation switch on and switch off by modulating TCR expression at the T cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection. Additionally, the ZAP70-dependent signaling pathway may also contribute to primary B cells formation and activation through the B cell receptor. Recruitment and activation of ZAP70 are transient and are terminated by phosphorylation of negative regulatory tyrosine residues and dephosphorylation of positively acting sites. | Additional information: Clone REA814 displays negligible binding to Fc receptors.