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TCR Vβ8.1, 8.2 Antibody, anti-

mouse, APC, REAfinity™, 30 µg in 1 mL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-109-026
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      TCR Vβ8.1, 8.2

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA585

    • 标记物

      APC

    • 适应物种

      mouse

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:10

    • 抗体英文名

      TCR Vβ8.1, 8.2 Antibody, anti-mouse, REAfinity™

    • 抗体名

      TCR Vβ8.1, 8.2 Antibody, anti-mouse, REAfinity™

    • 规格

      30 µg in 1 mL

    Identification and enumeration of TCR Vβ8.1, 8.2+ cells by flow cytometryClone REA585 recognizes the mouse Vβ8.1, 8.2 T cell receptor (TCR Vβ8.1, 8.2) but not Vβ8.3. Vβ8.1 and 8.2 are variants of the TCR β chain that associates with the TCR α chain. The TCR is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a disulfide-linked membrane-anchored heterodimeric glycoprotein normally consisting of the highly variable α and β chains. TCR Vβ8.1, 8.2 is expressed on approximately 20 % of peripheral T cells in thymus, spleen, and other lymphoid organs in different mice strains, such as Balb/c, AKR, C57BL/6, AKR/B10.A. | Additional information: Clone REA585 displays negligible binding to Fc receptors

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    图标文献和实验
    该产品被引用文献
    Haskins, K. et al. (1984) The antigen-specific, major histocompatibility complex-restricted receptor on T cells. VI. An antibody to a receptor allotype. J. Exp. Med. 160 (2): 452–471. | Kuchroo, V. K. et al. (1994) T cell receptor (TCR) usage determines disease susceptibility in experimental autoimmune encephalomyelitis: studies with TCR V beta 8.2 transgenic mice. J. Exp. Med. 179 (5): 1659–1664. | Mami-Chouaib, F. et al. (2002) Antitumor cytotoxic T-lymphocyte response in human lung carcinoma: identification of a tumor-associated antigen. Immunol. Rev. 188: 114–121.
    相关实验
    • APC和TSC-2 cDNA的长5'RACE

       图2 APC 5'末端的扩增。用对应APC mRNA的位置5846的GSP反转录1mg Hela总RNA得到APC mRNA。cDNA被纯化、加尾,和用对应位置1797(A带)和位置1632(B带)的引物和AAP一起,用ELONGASE酶混合物来扩增(94℃ 30s,接着35个循环的94℃ 15 s,55℃ 30 s,68℃ 5 min)。初步PCR用TE缓冲液稀释100倍,取1ml用引物APC-1632(A带)或引物APC-1242(B带)和AUAP分别重新扩增(30个循环),每个PCR

    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC Anti-Mouse Bead Kit 1)        Completely resuspend the AbC capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    • v468 chapter 5 糖原合成酶激酶-3 的抑制

      ), GSK-3b (dilution1:1,000; 1 h at RT), or b-actin (dilution 1:10,000; 1 h at RT). 5. Wash the membrane three times for 5 min each in TBS-T. 6. Incubate the membrane for 1 h at RT with 30 mL of TBS-Tcontaining anti-mouse or anti-rabbit IgG

    图标技术资料

    暂无技术资料 索取技术资料

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    文献支持
    TCR Vβ8.1, 8.2 Antibody, anti-mouse, APC, REAfinity™, 30 µg in 1 mL
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