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IL-4 Antibody, anti-human, PE,

REAfinity™, 100 tests in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-114-842
  • 2025年12月09日
  • Intracellular flow cytometry, Mass cytometry
  • human cell line
  • human, non-human primate, other
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      IL-4

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA895

    • 标记物

      PE

    • 适应物种

      human, non-human primate, other

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Intracellular flow cytometry, Mass cytometry

    • 浓度

      1:50

    • 抗体英文名

      IL-4 Antibody, anti-human, REAfinity™

    • 抗体名

      IL-4 Antibody, anti-human, REAfinity™

    • 规格

      100 tests in 200 µL

    Identification and enumeration of IL-4+ cells by flow cytometryClone REA895 recognizes the human interleukin-4 (IL-4), which is predominantly secreted by activated CD4+ memory and effector Tʜ2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The Tʜ2 types of immune mechanisms can also be involved in immunological disorders such as IgE-mediated allergy. | Additional information: Clone REA895 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Yokota, T. et al. (1986) Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities. Proc. Natl. Acad. Sci. U.S.A. 83 (16): 5894–5898. | Abrams, J. S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127: 5–24. | Brown, M. A. et al. (1997) Functions of IL-4 and control of its expression. Crit. Rev. Immunol. 17 (1): 1–32.
    相关实验
    • T-cell dependent antigen specific in situ staining (Pe)

      -conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining

    • AbC Anti-Mouse Bead Kit

      suspension. 5)        Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)        Add a pre-titrated

    • AbC Anti-Rat/Hamster Bead Kit

      1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)     

    图标技术资料

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    IL-4 Antibody, anti-human, PE, REAfinity™, 100 tests in 200 µL
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