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QA-2 Antibody, anti-mouse, FIT

C, REAfinity™, 30 µg in 1 mL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-107-906
  • 2025年12月09日
  • Flow cytometry
  • human cell line
  • mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      QA-2

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA523

    • 标记物

      FITC

    • 适应物种

      mouse

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry

    • 浓度

      1:10

    • 抗体英文名

      QA-2 Antibody, anti-mouse, REAfinity™

    • 抗体名

      QA-2 Antibody, anti-mouse, REAfinity™

    • 规格

      30 µg in 1 mL

    Identification and enumeration of QA-2+ cells by flow cytometryClone REA523 recognizes the mouse Qa-2 antigen, a 40 kDa single-pass type I membrane protein, which is also known as H-2 class I histocompatibility antigen Q7 alpha chain (H2-Q7). Qa-2 is a murine non-classical MHC class I molecule involved in the modulation of immune responses by interacting with T CD8+ and NK cell inhibitory receptors. The murine Qa-2 genes map to the H-2Q region, between the classical class I H-2D locus and the H-2TL cluster of class lb sequences. This locus encodes a variable number of class Ib genes in different strains. Strains expressing high (Qa-2high), medium (Qa-2med), low (Qa-2low), and no Qa-2 (Qa-2nul) were identified. Qa-2 is found as membrane-bound and soluble isoforms generated by alternative splicing. It is expressed mostly by lymphoid-derived cells and plays an important role in controlling growth and murine fetal development and, like HLA-G, the molecule is related to the protection of the fetus by inhibiting maternal NK cell-mediated lysis. | Additional information: Clone REA523 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Melo-Lima, B. L. et al. (2014) Differential transcript profiles of MHC class Ib(Qa-1, Qa-2, and Qa-10) and Aire genes during the ontogeny of thymus and other tissues. J Immunol: . | Devlin, J. J. et al. (1985) Duplicated gene pairs and alleles of class I genes in the Qa2 region of the murine major histocompatibility complex: a comparison. EMBO J. 4 (12): 3203–3207. | Lu, N. et al. (2011) Dynamic expression of Qa-2 during acute graft rejection. Mol. Med. 17 (3-4): 248–255.
    相关实验
    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC Anti-Mouse Bead Kit 1)        Completely resuspend the AbC capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    • Anti-GFP/BrdU In Situ Immunostaining

      Dilute 1 µL fresh streptavidin-Alexa Flour 555 in 1 mL PBS (1:1,000).  Spin the dilute antibody at high speed for 30'  to remove particulates.  Add supernatant to specimen and incubate 30' at room temp.  Remove antibody and add 1 mL PBS.  O.K

    • AbC Anti-Rat/Hamster Bead Kit

      1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)     

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    文献支持
    QA-2 Antibody, anti-mouse, FITC, REAfinity™, 30 µg in 1 mL
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