- 询价
- Miltenyi Biotec
- 德国
- 130-133-796
- 2025年12月09日
- Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), 3D-Immunofluorescence, Immunofluorescence, Immunohistochemistry, Mass cytometry
- human cell line
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Ki-67
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
REA183
- 标记物：
Vio G570
- 适应物种：
human, mouse
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), 3D-Immunofluorescence, Immunofluorescence, Immunohistochemistry, Mass cytometry
- 浓度：
1:50
- 抗体英文名：
Ki-67 Antibody, anti-human/mouse, REAfinity™
- 抗体名：
Ki-67 Antibody, anti-human/mouse, REAfinity™
- 规格：
600 µL
Detection of Ki-67+ cellsClone REA183 recognizes the Ki-67 antigen, a nuclear and nucleolar protein, which is strictly associated with cell proliferation. Two Ki-67 isoforms with molecular weight, 395 and 345 kDa are known. Both isoforms contain 16 “Ki-67 repeat“ sequences, each of which includes a conserved 66 bp “Ki-67 motif“. Expression of Ki-67 protein is present during all active phases of the cell cycle (G(1), S, G(2), and mitosis), but is absent from resting cells (G(0)). Due to strict association of Ki-67 with proliferating cells, Ki-67 is often used as an indicator of the “growth fraction“ of a given cell population. The N-terminal portion of Ki-67 contains a forkhead associated (FHA)1 domain, which is involved in interaction with proteins such as Hklp2 and NIFK. | Additional information: Clone REA183 displays negligible binding to Fc receptors. |
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文献和实验该产品被引用文献
Verheijen, R. et al. (1989) Ki-67 detects a nuclear matrix-associated proliferation-related antigen. II. Localization in mitotic cells and association with chromosomes. J. Cell. Sci. 92: 531–540. | Takagi, M. et al. (2001) A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis. J. Biol. Chem. 276: 25386–25391. | Bhatnagar, N. et al. (2017) Potential role of Vδ2+ γδ T cells in regulation of immune activation in primary HIV infection. Front Immunol 8: . | Campbell, S. et al. (2018) Myeloid cell recruitment versus local proliferation differentiates susceptibility from resistance to filarial infection. Elife 7: . | Schlöder, J. et al. (2017) Dimethyl fumarate therapy significantly improves the responsiveness of T cells in Multiple sclerosis patients for immunoregulation by regulatory T cells. Int J Mol Sci 18: E271. | Hyrenius-Wittsten, A. et al. (2018) De novo activating mutations drive clonal evolution and enhance clonal fitness in KMT2A-rearranged leukemia. Nat Commun 9 (1): 1770. | Jackson-Jones, L. H. et al. (2016) Fat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation. Nat Commun 7: 12651.
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Ki-67 Antibody, anti-human/mouse, Vio® G570, REAfinity™, 600 µL
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