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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
HLA-A3
- 亚型:
recombinant human IgG1
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,2-8℃
- 克隆性:
REA950
- 标记物:
Vio Bright V423
- 适应物种:
human
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
human cell line
- 应用范围:
Flow cytometry
- 浓度:
1:50
- 抗体英文名:
HLA-A3 Antibody, anti-human, REAfinity™
- 抗体名:
HLA-A3 Antibody, anti-human, REAfinity™
- 规格:
100 tests in 200 µL
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文献和实验MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
浓度为 1x107cells/ 100 μl,从中取 1 - 2x106 cells; 2. 用去离子水将 10xBuffer IS 稀释为 1x,准备 3 ml 稀释后的 Buffer IS; 3.取 1ul Strep-Jactin®PE 或 Strep-Tactin®APC,0.8μl MHC I-Strep,与 1x Buffer IS 混匀为 10ul 的终体积,冰上孵育 45 分钟; 4.将上一步孵育完的 Streptamers 复合物加入到细胞悬液中轻轻吹打混匀,冰上孵育 45 分钟
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
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