- 询价
- Miltenyi Biotec
- 德国
- 130-117-327
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- human cell line
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
H-2Kk
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
REA1029
- 标记物：
APC-Vio 770
- 适应物种：
mouse
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度：
1:50
- 抗体英文名：
H-2Kk Antibody, anti-mouse, REAfinity™
- 抗体名：
H-2Kk Antibody, anti-mouse, REAfinity™
- 规格：
30 µg in 200 µL
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文献和实验实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
Monoclonal Antibody Production
drip off the end of the tail until you have collected ~200 µl.(You may have to pre-bleed twice,with a week or so between bleeds). (5) Take the collected blood and place at 37 ℃ for 30 min.to remove complement. (6) Place blood at 4 ℃ overnight to clot
Anti-GFP/BrdU In Situ Immunostaining
Dilute 1 µL fresh streptavidin-Alexa Flour 555 in 1 mL PBS (1:1,000). Spin the dilute antibody at high speed for 30' to remove particulates. Add supernatant to specimen and incubate 30' at room temp. Remove antibody and add 1 mL PBS. O.K
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