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CD97 Antibody, anti-human, PE,

100 tests in 1 mL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-097-102
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Immunocytochemistry
  • mouse
  • human
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD97

    • 亚型

      mouse IgG1κ

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      VIM3b

    • 标记物

      PE

    • 适应物种

      human

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      mouse

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Immunocytochemistry

    • 浓度

      1:11

    • 靶点

      CD97

    • 抗体英文名

      CD97 Antibody, anti-human

    • 抗体名

      CD97 Antibody, anti-human

    • 规格

      100 tests in 1 mL

    Detection of human CD97+ cellsThe VIM3b monoclonal antibody reacts with human CD97, a member of the epidermal growth factor (EGF)-TM7 family of cell surface receptors. CD97 is expressed by various cell types of the hematopoietic system and also on smooth muscle cells. Highest constitutive expression is found on granulocytes and monocytes. Lymphocytes express CD97 at lower level (T and NK cells expressing higher levels than B cells) but on T cells CD97 is upregulated upon activation via CD3 and CD28 or PHA. CD97 is a ligand for CD55 (decay-accelerating factor, DAF) and has been shown to mediate cell adhesion and co-stimulation of T cell proliferation.

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    图标文献和实验
    相关实验
    • T-cell dependent antigen specific in situ staining (Pe)

      and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.

    • Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum

      Sample buffer, one 60‐ml bottle Wash buffer, phosphate‐saline concentrate, 5×, one 100 ml bottle: transfer contents to a graduated cylinder, bring volume up to 500 ml

    • Phycoerythrin conjugation protocol

      and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze.     Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD

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